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All Journal HAYATI Journal of Biosciences Jurnal Ilmu Pertanian Indonesia Zuriat BIOTROPIA - The Southeast Asian Journal of Tropical Biology Jurnal Agro JSDH Indonesian Journal of Science and Technology BERITA BIOLOGI JURNAL BIOLOGI INDONESIA Jurnal Bioteknologi & Biosains Indonesia (JBBI) Menara Perkebunan Jurnal Jamu Indonesia EKOTONIA: Jurnal Penelitian Biologi, Botani, Zoologi dan Mikrobiologi
Diah Ratnadewi
Department Of Biology, Faculty Of Mathematics And Natural Sciences, IPB University, Bogor 16680, Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Computer Science & IT Earth & Planetary Sciences Education Environmental Science Immunology & microbiology Medicine & Pharmacology Veterinary Other

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Journal : BIOTROPIA - The Southeast Asian Journal of Tropical Biology

 1 
LOCALIZATION OF GFDD4-1 EXPRESSED PROTEIN IN Physcomitrella patens CELLS DIAH RATNADEWI
BIOTROPIA - The Southeast Asian Journal of Tropical Biology Vol. 13 No. 1 (2006)
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (215.934 KB) | DOI: 10.11598/btb.2006.13.1.214

Abstract

The expression of a new dehydration-related gene of Physcomitrella patens, GFDD4-I, was traced for its localization in the plant cells. This revelation is useful to predict the possible roles of the protein in plant tolerance to environmental stress. This gene was fused to  gfp  marker gene and transfected into the plant protoplasts. Under a confocal laser microscope, it was detected that the GFDD4-1 protein associated with the OFF started to generate at the cell periphery and developed more intensively inwards to cytoplasm, forming vesicles and cystemal structures or network. The protein might be membrane protein which may involve directly in membrane maintenance or cellular protection against stress conditions. Key words : Protoplast transformation, protein localization, dehydration-related gene, GFP, Physcomitrella patens
Enhanced Production Level of Quinine in Cell Suspension Culture of Cinchona ledgeriana Moens by Paclobutrazol Diah Ratnadewi
BIOTROPIA - The Southeast Asian Journal of Tropical Biology Vol. 20 No. 1 (2013)
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (668.335 KB) | DOI: 10.11598/btb.2013.20.1.291

Abstract

Quinine is one of the major alkaloids in Cinchona spp., and it is used both as a medication and as a drink additive. The plant produces most of its alkaloids in the bark after 6–8 years of age.Repeated harvests can be performed until the plant dies, but only after every 3–5 years. We tested an improved method for culturing cell suspensions of Cinchona ledgerianato investigate the possibility of increasing production of quinine. The clone QRC 315 was treated with either a growth retardant (abscisic acid or paclobutrazol) or precursor feeding of L-tryptophan. To generate stress, we applied mannitol at 5.3 g/L combined with sucrose at a lower concentration (20 g/L),and we used sucrose at 30 g/L as the control. Paclobutrazol (7 mg/L) significantly suppressed cell growth and produced the highest level of quinine (11%) after 7 weeks of culture. L-tryptophan also reduced cell growth, but without any positive effect in the production of quinoline. The highest amount of quinine per culture flask, however,  resulted in cells treated with 3 mg/L abscisic acid.
IDENTIFICATION OF SECRETORY STRUCTURE, HISTOCHEMISTRY AND PHYTOCHEMICAL COMPOUNDS OF MEDICINAL PLANT Hyptis capitata Jacq. Darius Rupa; Yohana Caecilia Sulistyaningsih; Dorly Dorly; Diah Ratnadewi
BIOTROPIA - The Southeast Asian Journal of Tropical Biology Vol. 24 No. 2 (2017)
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11598/btb.2017.24.2.499

Abstract

Hyptis capitata Jacq. (common name: Knobweed or False Ironwort) belongs to Lamiaceae family and is among known traditional medicine. The Anak Dalam Tribe of Jambi Province uses the leaves of H. capitata to cure external and internal wounds. This study was aimed at identifying and analyzing secretory structure, histochemistry and phytochemical content of H. capitata leaves.  The results showed that H. capitata leaves have secretory structures in the form of peltate, capitate and uniseriate glandular trichomes on the upper and lower leaf surfaces, with idioblast cells scattered throughout the leaf mesophyll. Histochemical tests indicated that the peltate trichomes have four head cells, containing alkaloids and terpenoids. This study classified capitate trichomes into two types: 1. type I has one stalk cell and two head cells, all containing  alkaloids, terpenoids and lipophilic compounds; 2. type II has long stalk cells consisting of seven to ten cells with one head cell, all containing alkaloids and terpenoids. Uniseriate glandular trichomes of H. capitata leaves consist of four to eight cells containing alkaloids and terpenoids. The idioblast cells are round-shaped and contain lipophilic compounds. GC-MS analysis showed that H. capitata leaves  contain terpenoid compounds assumed to serve as anti-infective agents, including l-limonene, eugenol, farnesol isomers A, d-nerolidol, hexahydrofarnesol and neophytadiene.
Co-Authors . Suharyo . SUMARYONO . TRIVADILA Adabiyah, Rifatul Ai Nurhasanah Husnul Izzati Anggraini Barlian ARIS TJAHJOLEKSONO Aryani Leksonowati Atik Choirul Hidajah Christofora Hanny Widjaja Darius Rupa Dian Rahma Pratiwi Dorly Dorly Dyah Iswantini Dyah Retno Wulandari Efah FITRAMALA Elsa May Susanti ENDANG HADIPOENTYANTI Erwin Al Hafiizh Eva KHAERUNNISA Fitri Nur`Aeni Hadi SUNARSO Hari Basuki Notobroto Hayati MINARSIH Ika Dewi Ana Imron RIYADI Khalisa Aini Sinaga Leksonowati, Aryani Lilis Solihat Maulidiyah Utami Muhamad Ayi Pandu Perdana Nina Ratna Djuita Nur Amallia Nurmuliayanti Muis Rahmi, Putri Rifatul Adabiyah Rinny Saputri Rinny Saputri Rizka Musdalifah Amsar Soekisman Tjitrosoedirdjo Soekisman Tjitrosoedirdjo Soekisman Tjitrosoedirjo Sumaryono . Titiek Setyawati Titiek Setyawati Titiek Setyawati Tri Muji Ermayanti Tri Muji Ermayanti Tri Muji Ermayanti Triati Dewi Kencana Wungu Wijayanta, Ardian Nur WIRDHATUL MUSLIHATIN Witjaksono Witjaksono Witjaksono Witjaksono WOLFGANG FRANK Yohana Caecilia Sulistyaningsih Yustiny Andaliza Hasibuan Zainal Alim Mas’ud Zainal Alim Mas’ud